16JD – Impact of immune check point blockade on sphingolipid metabolism

Status: Available
Faculty Name: jessy-deshane-3
Primary Faculty Appointment: UAB
UAB/HA Department: Medicine
Campus Address: THT 433A, Department of Medicine/Pulmonary Allergy and Critical Care
Telephone Number: (205) 996-2041
Email: jessydeshane@uabmc.edu or Click to Send E-Mail
For how many summers have you served as a preceptor: 1
CCC Research Area: Cancer Biology & Immunology
Number of hours per week that the preceptor will personally supervise or work with the intern: 4
Other faculty, staff, or graduate students who may help to supervise the intern:
1. Kayla Goliwas
2. Jessy Deshane
Title of Project: 16JD – Impact of immune check point blockade on sphingolipid metabolism
Project Description:

Several studies have identified that dysregulation of sphingolipid metabolism occurs during lung cancer progression. The bioactive sphingolipids including, ceramide (Cer), sphingosine (So), and sphingosine-1-phosphate (S1P) are important in signaling and regulation of many cellular processes including cell proliferation, apoptosis, cellular senescence and angiogenesis associated with lung cancer. Specifically, the imbalance of anti-apoptotic S1P and pro-apoptotic Cer and So ratio regulated by sphingokinase (SK1 and SK2) enzymes contributes to these processes. Currently, it is unknown if immune check point blockade (ICB) therapy would modulate sphingolipid metabolism pathway. We have developed a three dimensional human lung tumor tissue model using a perfusion bioreactor platform. Using these models, we will investigate if the anti-apoptotic to pro-apoptotic sphinglipid ratios are altered by ICB. Samples will be analyzed by lipidomics and image analyses of expression data in tissues from the 3D-models. Data analyses can be done remotely following limited person training.

Project Status: Already up and running
Location of Project: Birmingham, AL (UAB)
Proposed Start Date: May 31, 2021
Proposed End Date: August 13, 2021
Expected work schedule for intern: Flexible, intern can largely set his or her own schedule (as for students who are instructed how to proceed and are permitted to work independently with weekly guidance) and should contribute full-time effort.
Number of days that the student will be expected to come physically to UAB:
1 day or a few days as needed
Category of Project: Clinical (Patient Care) Research
Cancer topic: Lung and Bronchus
Does this project involve human subjects: Yes
Does this project involve animal subjects: No

Receive training


Analyze data and plot


Meet by zoom weekly to discuss data

Preceptor will provide intern with access to the following: Laboratory work bench space
Likelihood that intern will be included as an author on one or more publications related to this summer research project: Very likely
Areas in which the ideal candidates will have experience: Biochemistry, Immunology, Pathology, Statistics and Data Management, basic knowledge