08JD – Exosomal signaling by myeloid cells in suppressing B cell responses in lung cancer

Status: Filled – Intern: Veena Krishnan
Intern: Veena Krishnan
Faculty Name: jessy-deshane
UAB Department: Medicine/Pulmonnary Allergy and Critical Care Medicine
UAB School: Medicine
Campus Address: THT 433A, Department of Medicine/Pulmonary Allergy and Critical Care
Telephone Number: (205) 382-0847
Email: jessydeshane@uabmc.edu or Click to Send E-Mail
For how many summers have you served as a preceptor: 1
CCC Research Area: Inflammation, Immunology, and Immunotherapeutics
Number of hours per week that the preceptor will personally supervise or work with the intern: 40
Other faculty, staff, or graduate students who may help to supervise the intern:
1. Yong Wang
2. Kayla Goliwas
Title of Project: 08JD – Exosomal signaling by myeloid cells in suppressing B cell responses in lung cancer
Project Description:

The tumor microenvironment (TME) plays a crucial role in regulating tumor biology and response to therapeutic intervention, with intercellular communication between tumor and stromal cells regulating tumor growth and progression. We recently reported that immunosuppressive myeloid-derived suppressor cells (MDSCs) suppress B cell responses in the lung TME. Using mouse models and human samples, we will investigate if MDSC-derived extracellular vesicles modulate sphingolipid signaling in B cells and contribute to dysregulation of B cell function in lung cancer. Additionally, we will investigate the role of exosomal mitochondria in class switching of B cells.

Project Status: Already up and running
Location of Project: Birmingham, AL (UAB)
Proposed Start Date: June 1, 2020
Proposed End Date: August 1, 2020
Expected work schedule for intern: Not very flexible, intern MUST be at work on certain days of the week and at certain times of the day (as may be necessary to interview patients, attend lab meetings, process samples, etc.) and should contribute full-time effort.
Category of Project: Animal Research
Cancer topic: Lung and Bronchus
Does this project involve human subjects: No
Does this project involve animal subjects: No

Perform co-cultures of tumor celld erived exosomes with B cells and Bcell-T cell co-cultures and analyse class switching and other functional aspects of B cells. A


Analyze internalization of exosomes by B cells


analyze and quantitate data and prepare poster presentation

Preceptor will provide intern with access to the following:
Office or desk space, Computer and printer, Laboratory work bench space, Supplies needed to complete project, Equipment needed to complete project
Likelihood that intern will be included as an author on one or more publications
related to this summer research project:
Very likely
Areas in which the ideal candidates will have experience:
Biochemistry, Cell Biology, Immunology, Statistics and Data Management, basic knowledge