12JD – Epithelial stem cells cross-talk with immune cells in the 3D- lung tumor microenvironment

Status: Filled – Intern: Carson Huynh
Intern: Carson Huynh
Faculty Name: jessy-deshane-2
UAB Department: Medicine
UAB School: medicine
Campus Address: THT 433A, Department of Medicine/Pulmonary Allergy and Critical Care
Telephone Number: (205) 382-0847
Email: jessydeshane@uabmc.edu or Click to Send E-Mail
For how many summers have you served as a preceptor: 1
CCC Research Area: Inflammation, Immunology, and Immunotherapeutics
Number of hours per week that the preceptor will personally supervise or work with the intern: 40
Other faculty, staff, or graduate students who may help to supervise the intern:
1. Kayla Goliwas
2. Yong Wang
Title of Project: 12JD – Epithelial stem cells cross-talk with immune cells in the 3D- lung tumor microenvironment
Project Description:

The tumor microenvironment (TME) plays a crucial role in regulating tumor biology and response to therapeutic intervention, with intercellular communication between tumor and stromal cells regulating tumor growth and progression. We have recently developed a three dimensional perfusion platform for culturing human lung tumor tissue. Using this platform, we have identified a unique population of epithelial stem cells that are positive for Keratin 17. We will use 3D-lung tumor model to investigate cross-talk of these epithelial stem cells with immune cells and fibroblasts in the lung tumor micrenvironment.

Project Status: Already up and running
Location of Project: Birmingham, AL (UAB)
Proposed Start Date: May 18, 2020
Proposed End Date: July 31, 2020
Expected work schedule for intern: Not very flexible, intern MUST be at work on certain days of the week and at certain times of the day (as may be necessary to interview patients, attend lab meetings, process samples, etc.) and should contribute full-time effort.
Category of Project: Laboratory Research
Cancer topic: Lung and Bronchus
Does this project involve human subjects: Yes
Does this project involve animal subjects: No

Immunohistochemical staining histomorphometric analyses


Quantitation of stem cells, fibroblasts and immune cells and evaluate changes in presenece of anti-PDL-1 antibody


Statsitcial analyses and plotting data and making poster

Preceptor will provide intern with access to the following:
Office or desk space, Computer and printer, Laboratory work bench space, Supplies needed to complete project, Equipment needed to complete project
Likelihood that intern will be included as an author on one or more publications
related to this summer research project:
Very likely
Areas in which the ideal candidates will have experience:
Cell Biology, Immunology, Laboratory Skills, basic knowledge, Statistics and Data Management, basic knowledge