JNEN January2010 cover HumanIVBNB
JNEN_January 2010-cover: Cover image of the January 2010 edition of the Journal of Neuropathology and Experimental Neurology announcing the first successful isolation, purification and characterization of human endoneurial endothelial cells that form the blood-nerve barrier.
Hydrailic Conductivity
Hydraulic-Conductivity: Equipment set-up to measure transendothelial fluid flux across the human in vitro blood-nerve barrier under a hydrostatic pressure gradient (hydraulic conductivity).
BNB Transcriptome
BNB-Transcriptome: Composite image shows transcript similarities and differences between cultured human endoneurial endothelial cells that form the in vitro blood-nerve barrier and laser captured microdissected endoneurial microvessels from normal adult sural nerves that form the in situ blood-nerve barrier.
Human TJ qRT PCR
Human TJ-qRT-pCR: Image shows bar histograms depicting tight junction molecular transcript fold-change following exogenous GDNF-mediated restoration of the human blood-nerve barrier after injury in vitro (green= upregulated; red= downregulated).
Proteomic Gel GDNF IVBNB 09 2018
Proteomic Gel-GDNF-IVBNB: Image shows an SDS-PAGE gel of the membrane and cytosolic fractions of cultured confluent human endoneurial endothelial cell monolayers with and without treatment with GDNF following injury, subsequently digested for proteomic analysis.
Junctional Complex IVBVB
Junctional-Complex-IVBNB: Composite mage shows the molecular effects of exogenous GDNF on restoring restrictive tight junction complex formation at the human in vitro blood-nerve barrier following injury compared to basal conditions.
Human BNB Validation Figure
Human-BNB-Validation-Figure: Composite mage demonstrates in situ protein expression of transcripts detected by whole exome shotgun sequencing of the human blood-nerve barrier in normal adult sural nerve biopsies
Leukocyte Trafficking GBS
Leukocyte Trafficking-GBS: Composite image demonstrates infiltration of CD11b+ mononuclear leukocyte in the sural nerve biopsies of three patients with the acute inflammatory demyelinating polyradiculoneuropathy variant of Guillain-Barré syndrome, associated with demyelination.
Leukocyte Trafficking BNB GBS
Leukocyte Trafficking-BNB-GBS: Composite image demonstrates CD11b+ mononuclear leukocyte trafficking across the blood-nerve barrier into the sural nerve endoneurium associated with demyelination and axonal loss in Guillain-Barré syndrome. Low and high magnification electron micrographs demonstrate intact endoneurial endothelial intercellular tight junctions during leukocyte trafficking in demyelinating Guillain-Barré syndrome.
Future Targets Immunotherapy GBS
Future-Targets-Immunotherapy-GBS: Image showing potential targets for specific immune molecule therapeutic intervention to treat demyelinating Guillain-Barre syndrome.
BNB CIDP
BNB-CIDP: High magnification image demonstrates intact endoneurial endothelial tight junctions associated with basement membrane duplication and perivascular T-lymphocyte infiltrates in a patient with chronic inflammatory demyelinating polyradiculoneuropathy.
Mouse BNB Day7 Crush
Mouse-BNB-Day 7-Crush: High magnification composite electron micrograph images of mouse endoneurial microvessels that form the BNB showing near-complete restoration of microvascular impermeability to horseradish peroxidase in the sciatic nerves of wildtype (WT) and heterozygous (HT) transgenic mice with normal GDNF expression compared to uninjured Sham control nerves. Increased mouse blood-nerve barrier permeability is seen in conditional GDNF knockout mice with abrogated sciatic nerve GDNF expression after injury.
Mouse BNB Day14 Crush
Mouse-BNB-Day 14-Crush: High magnification composite electron micrograph images of mouse endoneurial microvessels that form the BNB showing complete restoration of microvascular impermeability to horseradish peroxidase in the sciatic nerves of wildtype (WT) and heterozygous (HT) transgenic mice with normal GDNF expression compared to uninjured Sham control nerves. Persistently increased mouse blood-nerve barrier permeability is seen in conditional GDNF knockout mice with abrogated sciatic nerve GDNF expression after injury.