We use both mouse genetics and CRISPR/Cas9 based in vivo editing to achieve targeted gene knockout, knockin and modification.

(Left) Scheme of CRISPR-Cas9 based in vivo gene editing. (Right) Confocal image of a CRISPR edited mouse retina section stained with anti-HA antibody to visualize synaptic molecule Bassoon.


In vivo gene delivery using subretinal injection combined with in vivo electroporation.


Scheme of a generating conditional gene deletion mouse model using LoxP-Cre system.