Current research projects, disease and focus areas:

1.) Understanding the linkage between products of hemolysis, oxidative stress and matrix turnover leading to acute lung injury in the setting of sepsis, inhaled toxicant exposure and trauma

From Gaggar and Patel (2016)

Representative Citations:

o A Gaggar and RP Patel (2016) AJP-Lung Cellular and Molecular Physiology. “There is blood in the water: Hemolysis, hemoglobin, and heme in acute lung injury” 1;311(4):L714-L718

o Cornelia S Hahn, David W. Scott, Xin Xu, Mojtaba Abdulroda, Gregory A. Payne, J. Michael Wells, Liliana Viera, Colleen J. Winstead, Patricia L. Jackson, J. Edwin Blalock, Rakesh P. Patel*, Amit Gaggar (2015) The Matrikine N-α-PGP Couples Extracelllar Matrix Fragmentation to Endothelial Permeability. Science Advances 1:e1500175 PMCID: PMC4517288 *Co-Senior Author

o JY Oh, R Stapley, V Harper, M Marques, RP Patel (2015) Predicting storage-dependent damage to Red Blood Cells using nitrite oxidation kinetics, Peroxiredoxin-2 oxidation and hemoglobin / free heme measurements. Transfusion 12; 2967-78 PMCID: PMC4715702

o Joo-Yeun Oh, Jennifer Hamm, Xin Xu, Kristopher Genschmer, Ming Zhong, Jeffrey Lebensburger, Marisa B. Marques, Jeffrey D. Kerby, Jean-Francois-Pittet, Amit Gaggar, Rakesh P. Patel (2016). Absorbance and redox based approaches for measuring free heme and free hemoglobin in biological matrices Redox Biol. 167-177. doi: 10.1016/j.redox.2016.08.003. PMCID: PMC5007433

o #BM. Wagener, PJ Hu, JR. Richter, CA. Evans, J Honavar, AP. Brandon, JY Oh, J Creighton, SW. Stephens, MB. Marques, JD. Kerby, JF Pittet*, RP. Patel* (2018) Role of Heme in Lung Bacterial Infection after Trauma-Hemorrhage and stored RBC transfusion PLOS Medicine Mar 9;15(3):e1002522 PMCID: PMC5844517 *Co-senior authors

o Rekha Gautam, Joo-Yeun Oh, Marisa B. Marques, Richard A. Dluhy, Rakesh P. Patel (2018) Characterization of Storage induced Red Blood Cell hemolysis using Raman Spectroscopy. Laboratory Medicine 49(4): 298-310 PMCID: PMC6180846

o Allison R Jones, Rakesh P Patel, Marisa B Marques, John P Donnelly, Russell L Griffin, Jean-Francois Pittet, Jeffrey D Kerby, Shannon W Stephens, John R. Hess, Henry E Wang (2018) Older blood is associated with increased mortality and adverse events in massively transfused trauma patients: secondary analysis of the PROPPR trial. Critical Care Annals of Emergency Medicine. S0196-0644(18)31326

o Aggarwal S, Jilling T, Doran S, Ahmad I, Eagen JE, Gu S, Gillespie M, Albert CJ, Ford D, Oh JY, Patel RP, Matalon S. (2019) Phosgene inhalation causes hemolysis and acute lung injury. Toxicol Lett. 2019 S0378-4274(19)30104-3. doi: 10.1016/j.toxlet.2019.04.019. [Epub ahead of print]

o Oh JY, Marques MB, Xu X, Li J, Genschmer K, Gaggar A, Jansen JO, Holcomb JB, Francois-Pittet J, Patel RP (2020) Damage to Red Blood Cells during Whole Blood Storage J Trauma Acute Care Surg. [Epub ahead of print] PMID: 32301878

2.) Determine mechanisms by which nitrite mediates nitric oxide signaling in hypoxia and develop and test NO/nitrite-therapeutics to attenuate inflammatory disease

Representative Citations:

· JH Crawford, BK Chacko, HJ Pruitt, B Piknova, N Hogg, RP Patel (2004) Transduction of NO-bioactivity by the Red blood cell in Sepsis: Novel mechanisms of vasodilation during acute inflammatory disease. Blood. 104(5), 1375-82

· Jack H Crawford, James Huang, T. Scott Isbell, Sruti Shiva, Balu K Chacko, Alan Schechter, Victor M Darley-Usmar, Jeffrey D Kerby, John D Lang Jr, David Krauss, Chien Ho, Mark T Gladwin, Rakesh P Patel (2006) Hypoxia, Red Blood Cells and Nitrite Regulate NO-dependent Hypoxic Vasodilatation. Blood 107, 566-575

· Basu S, Grubina R, Huang J, Conradie J, Huang Z, Jeffers A, Jiang A, He X, Azarov I, Seibert R, Mehta A, Patel R, King SB, Hogg N, Ghosh A, Gladwin MT, Kim-Shapiro DB. (2007) Catalytic generation of N2O3 by the concerted nitrite reductase and anhydrase activity of hemoglobin. Nat Chem Biol. 3:785-794

· D. Vitturi, X Teng, JC Toledo, S Matalon, JR Lancaster Jr, RP Patel (2009) Effects of Hemoglobin Fractional Saturation on Nitrite transport in Red Blood Cells: Evidence for Hypoxic Regulation of Nitrite Export. Am J Physiol heart Circ Physiol. 296(5): H1398-407 PMCID PMC2685350

· C. Rodriguez, D. Vitturi, J He, M Vandromme, A Brandon, A Hutchings, LW Rue III, JD Kerby, RP Patel (2009) Sodium nitrite therapy attenuates hypertensive effects of HBOC-201 via a nitrite reduction. Biochem J 422(3), 423-432 PMCID PMC2775055

· Kelpke SS, Chen B, Bradley KM, Teng X, Chumley P, Moore B, Head H, Viera L, Thompson JA, Crossman DK, Bray MS, Eckhoff DE, Agarwal A, Patel RP (2012). Sodium nitrite protects against kidney injury induced by brain death and improves post transplant function. Kidney International. 82(3):304-13 PMCID: 3412933

· John D Lang Jr, Alvin B Smith, Angela Brandon, Kelley M Bradley, Yuliang Liu,D. Ralph Crowe, Nirag Jhala, Richard C Cross, Luc Frenette, Kenneth Martay, Youri Vater1, Alexander Vitin, Gregory Dembo, Derek DuBay, J Steven Bynon, Jeff M. Szychowski, Jorge Reyes, Jeffrey Halldorson, Stephen Rayhill, Andre Dick, Ramasamy Bakthavatsalam, Jo Ann Broeckel-Elrod1, Laura Sisson-Roberts, Terry Jordan, Lucinda Chen, Arunotai Siriussawakul, Devin E Eckhoff and Rakesh P. Patel (2014) Inhaled Nitric Oxide Administered Preemptively Improves Allograft Function and Decreases Post-operative Complications in Human Liver Transplantation PLOS One 9, e86053 PMCID: PMC3922702

· Jaideep Honavar, Stephen Doran, Joo Yeon Oh, Chad Steele, Sadis Matalon, Rakesh P. Patel (2014) Nitrite therapy improves post-chlorine gas exposure survival. AJP Lung Cell Mol Physiol 307:L888 PMCID: PMC4254962

· Anthony W. DeMartino, Daniel Kim-Shapiro, Rakesh P. Patel, and Mark T. Gladwin. Nitrite and nitrate chemical biology and signaling (2019). Brit. J. Pharmacology. Jan;176(2):228-245 PMCID: PMC6295445

· Sun CW, Yang J, Kleschyov A, Zhuge Z, Carlstrom M, Pernow J, Wajih N, Isbell TS, Oh JY, Cabrales P, Tsai A, Townes T, Kim-Shapiro DB, Patel RP*, Lundberg JO. (2019) Hemoglobin β93 Cysteine is Not Required for Export of Nitric Oxide Bioactivity From the Red Blood Cell. Circulation. Circulation. 2019 Jun 4;139(23):2654-2663 *Co-Corresponding Author PMCID: PMC6546526

3.) Determining how the oral microbiome is modulated by environmental stressors, that in turn regulates systemic and pulmonary nitric oxide bioavailability including lung injury and development in the newborn

Representative Citations:

· Nichols, KA Ahmed, RP Patel (2016) Yes to “NO” host flora symbiosis. The Biochemist 38, 5, 18-21

· K Ahmed, A Nichols, J Honavar, MT Dransfield, S Matalon, RP Patel (2017) Measuring nitrate reductase activity from human and rodent tongues Nitric oxide Jun 1;66:62-70

· #Joshipura KJ, Muñoz-Torres FJ, Morou-Bermudez E, Patel RP. (2017). Over-the-counter mouthwash use and risk of pre-diabetes/diabetes. Nitric Oxide 71:14-20

· Kaumudi Joshipura, Francisco Muñoz-Torres, Jeanpaul Fernández-Santiago, Rakesh P. Patel, Angel Lopez-Candales (2020) Over-the-counter mouthwash use, Nitric oxide and hypertension risk. Blood Press. Blood Press. 2020 Apr;29(2):103-112.

· Gentle SJ, Freeman A, Patel RP, Ambalavanan N, Lal CV.(2020) Airway nitrite is increased in extremely preterm infants with bronchopulmonary dysplasia Respir Res. 2020 Sep 21;21(1):244. doi: 10.1186/s12931-020-01508-8. PMID: 32957939

4.) Determine the role of redox mediators and endothelial N-glycans to regulate immune cell trafficking in atherosclerosis

Representative Citations:

o DW. Scott, J Chen, BK. Chacko, JG. Traylor Jr, AW Orr, RP Patel (2012) Role for endothelial N-glycan mannose residues in monocyte recruitment during atherogenesis. ATVB. 32(8): e51-9 doi:10.1161/ATVBAHA PMID: 22723438

o DW Scott, TS Dunn, ME Ballastas, SH Litovsky, RP Patel (2013) Identification of a high mannose ICAM-1 glycoform: effects of ICAM-1 hypoglycosylation on monocyte adhesion and outside in signaling. AJP-Cell. 305; C228 PMCID: PMC3725629

o DW Scott and RP Patel (2013) Endothelial heterogeneity and adhesion molecules N-glycosylation: implications in leukocyte trafficking in inflammation Glycobiology 23(6): 622

o Kellie Regal-McDonald, Brittney Xu, Jarrod Barnes, Rakesh P. Patel (2019) High-mannose intercellular adhesion molecule-1 (ICAM-1) enhances CD16+ monocyte adhesion to the endothelium. Am. J Phys Heart, Circ. Phys. 317(5):H1028-H1038 PMCID: PMC6879925

o Kellie Regal-McDonald, Maheshika Somarathna, Timmy Lee, Silvio Litovsky, Jarrod Barnes, J.M. Peretik, J.G. Traylor Jr., A. Wayne Orr, Rakesh P. Patel (2020) Assessment of high mannose and complex N-glycoforms of ICAM-1 in Mouse and Human Models of Endothelial Dysfunction PLoS One. 2020 Mar 24;15(3): e0230358. doi: 10.1371/journal.pone.0230358. eCollection 2020. PMID: 32208424

o Kellie Regal-McDonald, Rakesh P. Patel (2020). Sweet and sticky: selective recruitment of monocyte subsets by endothelial N-glycans. Am J Pathol. 2020 May;190(5):947-957. PMCID: PMC7221295

o McDonald KR, Hernandez-Nichols AL, Barnes JW, Patel RP (2020) Hydrogen peroxide regulates endothelial surface N-glycoforms to control inflammatory monocyte rolling and adhesion. Redox Biol. 2020 Jul;34:101498. doi: 10.1016/j.redox.2020.101498