1. Lanman, J., Lam, T., Barnes, S., Sakalian, M., Emmett, M.R., Marshall, A.G., and Prevelige, P.E., Jr.
    Identification of novel interactions in HIV-1 capsid protein assembly
    by high resolution mass spectrometry.
    Journal of Molecular Biology, 325:759-772, 2003.
        This piece of work concerns the folding of the capsid protein in the HIV-1 virus. A critical feature of this study was the use of high resolution FT-ICR mass spec to rapidly measure deuterium isotope enrichment in pepsin-derived capsid protein peptides.
  2. Aslan, M, Barnes, S., Kirk, M, Rosenfeld, S, Townes, T, Ryan, T and BA Freeman.
    Nitric oxide-dependent generation of reactive species in sickle cell
    disease: renal and hepatocellular actin tyrosine nitration.
    Journal of Biological Chemistry, 278: 4194-4204, 2003.
    Journal of Biological Chemistry, accepted for publication .
        In this piece of research from Dr. Bruce Freeman’s laboratory and carried out by Dr. Mutay Aslan, a ombination of MALDI peptide fingerprinting and anti-nitrotyrosine antibody immunoaffinity isolation of nitrated proteins, followed by peptide sequencing using LC-MSMS, was used for the first time to identify several specific sites of actin nitration that occur in vivo.
  3. Prasain, J.K., Jones, K., Kirk, M., Wilson, L., Smith-Johnson, M., Weaver, C.M., Barnes, S.
    Profiling and Quantification of Isoflavonoids in Kudzu Dietary
    Supplements by High-Performance Liquid Chromatography and
    Electrospray Ionization Tandem Mass Spectrometry.
    Journal of Agricultural Food Chemistry, 51:4213-4218, 2003.
        This paper explored the isoflavonoid content of dietary supplements based on the root of the Kudzu, a Japanese vine that is found covering the ground and often trees in the Deep South. The key issue was the demonstration that the principal isoflavones was puerarin, a 8-C-glucoside of daidzein. It is has a unique tandem mass spectrum compared to O-linked glucosides.
  4. Prasain, J.K., Boersma, B.J., Kirk, M., Wilson, L., Patel, R., Darley-Usmar, V.M., Botting, N. and Barnes, S.
    Mass spectrometric methods for the analysis of chlorinated and nitrated isoflavonoids: a novel class of biological metabolites.
    Journal of Mass Spectrometry, 38: 764-771, 2003.
        This paper examines the tandem mass spectra of isoflavone derivatives (chlorinated and nitrated) formed by reaction with reactive oxygen species.
  5. Carballal, S., Radi, R., Kirk, M.C., Barnes, S., Freeman, B.A. and Alvarez, B.
    Sulfenic acid formation in human serum albumin by hydrogen peroxide
    and peroxynitrite.
    Biochemistry, 42: 9906-9914, 2003.
        This paper utilized LC-tandem mass spectrometry to identify the oxidation of a critical cysteine residue on albumin by oxidizing agents, including peroxynitrite.
  6. Boersma, B.J., D’Alessandro, T., Benton, M.R., Kirk, M., Wilson, L.S., Prasain, J., Botting, N.P., Barnes, S., Darley-Usmar, V.M. and Patel, R.P.
    Neutrophil myeloperoxidase chlorinates soy isoflavones and enhances
    their antioxidant properties.
    Free Radical Biology and Medicine, 35:1417-1430, 2003.
        This paper demonstrates sing LC-MS that HL-60 cells differentiated with DMSO to form neutrophil-like cells rapidly and completely convert isoflavones to their chlorinated metabolites. These metabolites have greater antioxidant properties in Cu-stimulated LDL oxidation assays.
  7. D’Alessandro, T., Prasain, J., Botting, N.P, Moore, R., Darley-Usmar, V.M., Patel, R.P., and Barnes, S.
    Polyphenols, inflammatory response, and cancer prevention: chlorination
    of isoflavones by human neutrophils.
    Journal of Nutrition,133:3773S-3777S, 2003.
        The principal point of this paper was the demonstration using LC-MS that freshly isolated human neutrophils when stimulated convert the isoflavone genistein to chlorinated metabolites.
  8. He, D., Barnes, S, Falany, C.N.
    Rat liver bile acid CoA:amino acid N-acyltransferase
    : expression, characterization, and peroxisomal localization
    Journal of Lipid Research, on line September 1, 2003.
  9. Page G.P., Edwards J.W., Barnes S., and Allison, D.B.
    A Design and Statistical Perspective on Microarray Gene Expression Studies in Nutrition: The Need for Playful Creativity and Scientific Hard-Mindedness.
    Nutrition, 19: 997-1000, 2003.