Engineered cell lines and tissue patches

Our lab generates customized cell lines for daily research, including human-induced pluripotent stem cell (hiPSC) obtained from a human patient, multiple hiPSC-derived cardiac cell lines, and organoids synthesized from cardiac cells. These cell lines are genetically modified and can be used in many different projects. They may be transferred to collaborators to be used for research-only purposes. The lab PI (Dr. Zhang) has the final decision on how these cell lines and tissue patches can be transferred and used by other labs. The following cell lines, organoids, and tissues are available

– (to be updated)

Bioinformatics pipelines

Our lab also builds bioinformatics and statistics tools to analyze genetic data. These pipelines are freely available for public use. They include:

– Non-parametric test for very small (n=3 or n=4) sample group.

– Artificial Intelligence-based Autoencoder to cluster cells from single-cell RNA sequencing (scRNAseq) data. See more

– Artificial-Intelligence-based Sparse model analysis to quantify pathway/biological enrichment analysis in scRNAseq data. See more

Genetic datasets

According to the National Institute of Health recommendation, the following genetic data are deposited into the Gene Expression Omnibus, and freely available for public usage:

– GSE185289: Single nucleus transcriptomics: Apical resection in newborn pigs extends the time window of cardiomyocyte proliferation and myocardial regeneration.

– GSE144883: Bulk-RNA sequencing cardiac tissue; Identifying the Key Regulators that Promote Cell-cycle Activity in the Hearts of Early Neonatal Pigs after Myocardial Injury.